Flow Facility
Flow Facility
The Core Flow cytometry Facility (CFF) provides state-of-the-art flow cytometry analysis equipment and high-speed cell sorting with certification of ISO 15189 (M056) and ISO 9001 which makes it possible for research and diagnostics to work shoulder to shoulder.
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The CFF is currently equipped with:
- 6 FACS canto II, 3 lasers (405nm, 488nm, 635nm) which can detect 8 fluorescent parameters.
- One LSR II, 3 lasers (405nm, 488nm, 635nm) which can detect 10 fluorescent parameters.
- One LSR Fortessa (ISO 9001 only), 4 lasers (405nm, 488nm, 561nm, 635nm) which can detect 16 fluorescent parameters.
- One Aria II, 3 lasers (405nm, 488nm, 635nm) which can detect 10 fluorescent parameters and can sort and collect 4 different cell populations simultaneously.
- One Aria III (ISO 9001 only), 4 lasers (405nm, 488nm, 561nm, 635nm) which can detect 16 fluorescent parameters and can sort and collect 4 different cell populations simultaneously.
Cell sorting is done for:
- Next Gen sequencing
- Cell cloning (96 wells plate)
- Purifying Genetic modified cells
- Isolation of rare events
Sorting and measuring conditions:
- High speed sorting
- High viability sorting
- Sorting/analyzing at lab-, BSL 1-and BSL 2-level
- Index cell sorting
- High throughput analysis (96, 384 wells)
- Up to 18 parameter cell analysis
- Minimal residual disease detection (1 of 1*106)
All analyzers and high speed cell sorters have uniformly standardized cytometer settings, for different sizes of cells, based on several years of diagnostic and research immunofenotypic knowledge. This procedure makes it possible to participate in multi center trails.
The procedure of standardization is done as described in:
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols--T Kalina, J Flores-Montero, VHJ van der Velden et all.